Inhibition of biofilm growth of Gram-positive and Gram-negative bacteria on tuned polyurethane nanofibers

Biofilm formation is a process of bacterial attachment whereby they fasten irreversibly to biomaterial surface and lead unwanted phenotypic changes. The chief concern its prevent the harmful changes that follow accumulation bacteria on implants, so scientific community has made efforts. In this study, we attempted fabricate novel tissue engineering candidate biofilm desired by ideal biomaterials. We prepared micro/nanofibers polyurethane (PU) incorporated with hydrophilic β-cyclodextrin (CD) electrospinning technique. Further on, these as-spun fibers were in fused an antibacterial agent. As agent, silver nanoparticles (Ag NPs) adsorbed scaffolds. Among varied methods adsorption, adsorption sonication hydrothermal chosen. Characterization studies performed scanning electron microscopy (SEM) water contact angle analysis. uniform morphology nanofibers was seen SEM micrographs which mimics extracellular matrix. hydrophilicity test showed increased scaffolds decrease CD Ag NPs fibre release assay slow case where treatment compared sonication. tests show inhibition different degrees fibers. highest zones samples vitro MTT presented non-toxic cells could be employed biological applications.